Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis cercosporin quantification obtained by spectrophotometry (SPEC) high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities Brazil. Image crystals either high or low production the toxin. Our results show SPEC HPLC are equally valid evaluation C. cultures grown vitro. The had higher crystal number size when compared one production.